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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen 0.5 mM L-glutamine, and 5mM cytosme-D-arabinofuranoside from Sigma added at 2 days in vitro
For lean individuals, regulatory and immunosuppressive cells promote the clearance of dead adipose tissue, suppressing adipose tissue cell proliferation, and secreting anti-inflammatory cytokines (IL-10, IL-4, IL-13, and IL-1R) (58)
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This overview is intended to contextualize existing findings, without suggesting subtype-specific therapeutic applications (Table 1)