Gut Microbes (2020) 12(1):1801944
doi: 10.1023/A:1006841228483 28 Friedmann AngeliJPSchneiderMPronethBTyurinaYYTyurinVAHammondVJet al
5 and 6, samples in SDS solubilizer (0.0625 M TrisHCl (pH 6.8), 10% glycerol, 0.01% bromophenol blue, 2% (wt/vol) SDS, +/- 2% 2-mercaptoethanol) were heated at 95 C for 5 min, electrophoresed through 8% or 10% (wt/vol) polyacrylamide-SDS gels, transferred to nitrocellulose membranes (Bio-Rad, USA), and incubated with SARS-CoV-2 (2019-nCoV) S antibody rabbit PAb antigen affinity purified, mouse monoclonal anti-SARS-S2 conjugated to HRP, goat anti-human IgG-HRP, ACE2 recombinant rabbit monoclonal antibody, or purified LgBiT-substrate cocktail
Proceedings of the National Academy of Sciences of the United States of America, 94 (5), 1967
All required injection supplies were included, with extra sharps and alcohol wipes
NAD(+) and axon degeneration revisited: Nmnat 1 cannot substitute for Wld(S) to delay Wallerian degeneration