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Van Kampen-Zijlstras reagent was then added before measuring the absorbance of the supernatants at 540 nm using a microplate reader

Such organelle damage can trigger apoptosis and hepatocyte cell death

However, since a diagnosis of apoptosis implies a specific sequence of biochemical and morphological events and should ideally be supported by electron microscopy, it may be more prudent to diagnose single cell necrosis unless there is definitive proof of apoptosis provided by electron microscopy (Levin et al

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doi: 10.1046/j.1365-2133.2003.05478.x 57 de HeideLJMLaskewitzAJApersJA

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