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Because GSH status is dynamic, the outcome measures were extended beyond GSH status alone to include 8-OHdG and F2-isoprostanes (F2-isoP), in case a change in oxidation status was exemplified by evidence of reduced oxidation products rather than a change in GSH or GSH:GSSG ratio (i.e., improvement in tissue-level oxidative stress not represented by RBC indices)

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The methyl group is converted into formaldehyde

With the rise of Arabidopsis as a study system, and notably genome sequencing, the pathway and its genes were characterized at the molecular genetic level, revealing (or confirming) that all four enzymes were encoded by more than one gene, thus explaining the presence of the enzyme activities in several compartments (Table 1)

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