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These may represent aggregated peptide that cannot be restored to functional form, or they may indicate contamination

For highly hydrophobic peptides, dissolving the powder in a small volume of DMSO (dimethyl sulfoxide) before adding water often solves the issue

[DOI] [PMC free article] [PubMed] [Google Scholar] 68.Deb P., Chini A., Guha P., Rishi A., Bhan A., Brady B., Perrotti L.I., Mandal S.S

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5) Reducing Stress In a cell-based study, the stress hormone cortisol inhibited GH secretion [48]