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The bottom layer (chloroform phase) was collected for lipidomics analysis using LC-MS on an Orbitrap Exactive (Thermo Scientific) in line with an Ultimate 3000 LC (Thermo Scientific)

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He creates new things in order to make his life easier

Tobacco-derived nicotine is extracted from the tobacco plant

In the wake of the Surgeon General's 1964 report, Koop said that he "frequently spoke of the sleazy behavior of the tobacco industry in its attempts to discredit legitimate science as part of its overall effort to create controversy and doubt [surrounding the relationship between smoking and lung cancer]." The word "doubt" carried singular meaning in tobacco-policy circles, having been used in an infamous 1969 memo circulated within Brown and Williamson Tobacco Company, the maker of Pall Mall