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Analysis of protein expression by flow cytometry Cells were fixed in 4% paraformaldehyde in PBS (pH 7.4) for 20 min at room temperature and permeabilized in 0.1% Triton X-100 in PBS with 0.1% FBS for 5 min

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The novel makes liberal use of the historical details surrounding William Shakespeare's life, and exposes students to the possibility raised by some theorists that Edward de Vere, Earl of Oxford, was the true author of the works that have long been attributed to the Bard

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