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All of the above constructs were generated in pFastBac and pcDNA3.1 vectors for protein expression in insect cells and functional assays in mammalian cells, respectively

One important caveat

This is unusually broad for a topical skincare ingredient

The most common triggers are eating too much at one sitting, eating high-fat or greasy foods, eating too quickly, and lying down shortly after a meal

Much of this is reduced food volume and water weight rather than significant fat loss