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In the study by Gulati et al., the limitation lies in the fact that the relationship between smoking and post-operative complications in lumbar DDD patients was unable to be confirmed as causal, as potential confounding factors may play a role, which they had been unable to establish (Gulati et al., 2015)

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Among the few other existing microfluidic systems 32,33,34 , the majority rely on submerged CSE treatments, which fail to mimic physiologically relevant airliquid interface (ALI) conditions and dynamic mechanical forces present in the lung