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In 2004, the prevalence of current smoking among adults in 49 states, the District of Columbia, Puerto Rico, and the Virgin Islands was estimated by using data from the Behavior Risk Factor Surveillance Survey (CDC 2005c)

Health minister Frank Vandenbroucke said the inexpensive e-cigarettes had turned into a health threat since they are an easy way for teenagers to be drawn into smoking and get hooked on nicotine

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High-profit margins by the local retailers, the craze of the local youths for foreign cigarette brands at cheaper rates, the tempting flavour of some of the cigarette brands, etc

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Together, future studies on mtDNA methylation could further investigate whether pyrosequencing readout represents the methylation on mitochondrial genome or the accessibility of bisulfite conversion to mtDNA molecules which could be hampered by DNA-surrounding proteins such as TFAM