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Furthermore, syphilis often does not present any symptoms after infection, making it difficult to control its spread

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Soft tissue excision (5 mm in diameter, 3 mm thick) was then performed with a sterile scalpel, and tissue samples were placed in Eppendorf tubes (5.0 ml, Sigma-Aldrich, Darmstadt, Germany) with formaldehyde solution at a ratio of 1:15