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Using this as a baseline, the researchers added 70 live ticks to 10 of the fresh nest linings

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For Glycolytic Rate Assay, the medium was exchanged to XF DMEM medium (Agilent Technologies) with 2 mM glutamine, 10 mM glucose, 1 mM pyruvate, and 5 mM HEPES and incubated at 37 C without CO 2 1 h prior to the assay

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