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Differentially expressed genes (DEGs) using |log 2 (Fold Change)| 1 and padj 0.05 as threshold were obtained using DESeq2 (1.20.0) software

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Two technical developments paved the way for the dominance of the cigarette: first, the invention of flue curing, or drying tobacco at higher temperatures, which produced a golden yellow leaf with much milder smoke

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