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100 L of diluted NF-kappaB antibody (1:1,000 dilution in 1X Antibody Binding Buffer) was added to each of the 32 wells

While nicotine is not considered a classic carcinogen in the same category as tobacco smoke, it may promote tumor growth, blood vessel formation and cellular signaling pathways that support cancer progression

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The column was then washed at 500 L/min for 8 min at 100% mobile phase B followed by re-equilibrated at 0% mobile phase B, 500 L/min for 7 min

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