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Cells were then treated on the apical surface with FITC labeled poly I:C (0.5 or 2.5g/mL as indicated in figure legends), AF488-BSA (50g/mL), AF488 or AF594-CtxB (10g/mL), BODIPY-LacCer (1uM), AF488-Tfn (125g/mL) or FITC-Dextran (250g/mL) for the indicated times