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In brief, lung tissues or fibroblasts were homogenized with 1 Pre-Lysis Buffer and centrifuged at 1000 g for 5 min at 4 C

References - Heck, C

The genetic aspects of aging-related cataract have been discussed in depth in a recent review by Shiels and Hejtmancik 7 and in Chapter 74 of this work 11 Nuclear Cataract Nuclear cataracts are the most common form of aging-related lens opacity

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These modifications can change the structure of protein and produce new epitopes, which may not be covered by central tolerance, so they can trigger immune response under certain conditions

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