Dissected cochlear epithelia are transferred to permeable membrane inserts (Millicell organotypic culture plate inserts, Millipore) which are placed into 1 mL of culture media (Dulbecco's modified Eagle's medium (high glucose, Glutamax, 25 mM HEPES) with 10% fetal bovine serum, 1% N2 supplement and 10 units/ml penicillin) in a 6-well plate
Some investigators analyze whole-brain homogenates, whereas others focus on specific regions such as cortex, hippocampus, or cerebellum, each characterized by distinct redox and metabolic profiles
As cardiovascular disease is a leading cause of mortality associated with obesity, GLP-1's ability to reduce weight and enhance cardiovascular health is a pivotal step toward a healthier future
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