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A major reason was the prevailing culture of the time

Cleaved Cas3 + apoptotic cells were more frequent on day 7 and somewhat more frequent on days 1 and 14 after final CSS administration

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For the quantification of chlorophyll a (chl a) and chlorophyll b (chl b), the supernatant obtained after centrifugation was analyzed using a spectrophotometer at wavelengths of 663 nm and 645 nm, respectively