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Overexpression efficiency was detected by Western blotting (Supplementary Fig

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The supernatant was then mixed with 100 l Protein G magnetic beads bound to 10 l anti-PDHc-E2 or anti-OGDHc-E2 antibody and immunoprecipitated at 4 C for 16 h

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However, this has not always been the case and it has taken 2 changes in legislation to enable water to be distributed to drug users