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Enzyme-linked immunosorbent assay (ELISA) Serum samples from control and emphysema mice were collected and centrifuged at 2500g for 10 min

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The mean (SD) ages of the Y1-Y3 samples were 38.5 (9.7), 39.7 (10.1), and 39.9 (9.9) years, respectively, and in each of the samples there were more men (Y1-Y3: 81.6%, 80.3%, and 79.9%) than women (Y1-Y3: 18.4%, 19.7%, and 20.1%)