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HBECs were then incubated overnight with a primary antibody raised in rabbit against amino acids 427441 amino acid residues of human -tubulin (Rockland antibodies and assays, 600-401-880) to stain surface apical cilia and followed by staining with goat anti rabbit Dylight 649 secondary antibody (Vector laboratories, DI-1649) and DAPI

It took me 3 tries to figure out what I was doing wrong and to get into the rhythm of making these

They're genuinely different experiences, and choosing between them should come down to what you're actually after rather than convention or social pressure