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ALI cultures were washed with warm 1 PBS (5 min, 37 C), fixed in PFA (1 PBS, 3.2% PFA, 3% sucrose) for 20 min on ice and washed twice with ice cold PBS

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Inflammation and cancer Many cancers arise in areas of chronic inflammation, which plays a major role in tumor invasion, progression, and metastasis, largely through the cytokine-mediated activation of mechanisms involved in tissue repair, cell proliferation and angiogenesis [20, 22, 51,52,53]

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