c) coupling, optionally protected Aib and His amino acid, d) acylating an epsilon amino group of lysine residue in semapeptide with an acylating agent of Formula (V), which is optionally activated, wherein, Ri is hydroxy or a reactive ester thereof, R2 is selected from H or C1-12 alkyl
Claim 2: The process according to claim 1, wherein the said insoluble tag is optionally linked to a nucleotide sequence encoding affinity tag of Formula (III), Affinity Tag Insoluble tag Fusion tag Arg 34 GLP-l (9-37) Formula (III) Claim 3: The process according to claim 2, wherein the affinity tag is selected from Polyarginine-tag (Arg-tag), Polyhistidine-tag (His-tag), S-tag, SBP-tag (streptavidinbinding peptide), Maltose binding protein and chitin binding domain (CBD)
We did use the same protocol to also make semaglutide
For semaglutide, a peptide precursor backbone is recombinantly expressed in yeast, which is then modified with an N-terminal fragment (which includes one noncanonical amino acid) and a fatty acid linker
Semaglutide (link: is a drug that belongs to the class of insulin-like peptide-1 receptor agonists (GLP-1 RA)
The present application also relates to a recombinant process for the preparation of semapeptide
