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(D) Western blots were performed, and (E) the relative protein levels of Sp1, SIRT1, acetyl-HIF-1, HIF-1, p21, and p27 in MLE-12 cells were determined (n = 3)

The MPs abundance in farmed soil in Wuxi and Nanjing ranged from 420 to 1290 items/kg 26 , while in Hangzhou Bay, southeast China, the abundance was 263 items/kg in unmulched land and 571 items/kg in mulched land 104

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