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For TUNEL IF detection, slides were incubated with lectin PNA, Alexa Fluor 594 conjugate (Invitrogen, L32459) at room temperature for 1 h in the dark, and then treated with One Step TUNEL Apoptosis Assay Kit (Beyotime, C1088)
He said that he pointed out to the audience in all his speeches that it is no longer a matter of if but only a matter of when the transition will happen on a much broader scale
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