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Dilute aqueous ammonia (1 to 10%): for strongly acidic sequences that need a mildly basic environment

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The data emerging from the most recent reviews and meta-analyses on vitamin D and vitamin B12 in relation to thyroid function and autoimmunity are summarized in Table 1

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Following, the cell pellets, containing a known number of cells (~ 1 10 6 cells/mL), were dissolved in 1 mL reagent: 1 N sodium hydroxide (NaOH) and 10% DMSO, and boiled at 60 C for 30 min