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Experimental design figures were generated using images provided by Servier Medical Art under the Creative Commons Attribution 3.0 Unported License ( 3 Results 3.1 Acute smoke exposure triggers neutrophilic inflammation and occurrence of a unique alveolar macrophage subset in the lung To examine the effect of acute smoke exposure (4 days) on the pulmonary immune cell types and their molecular mechanisms, we performed scRNA-seq on cells collected by bronchoalveolar lavage from the alveolar space and from lung tissue of the same CS-exposed (n = 11) and air control (n = 7) animals ( Figure 2A )