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A: The dosage should be followed as per the doctors instructions

Mantovani G, Macci A, Madeddu C, Mura L, Massa E, Gramignano G, et al

J Atheroscler Thromb 2023;30:74-86.PubMed 76

After incubation (10 min, 39C), the plates were centrifuged (200 x g, 5 min, 20C, Universal 320, Hettich, Tuttlingen, Germany) and the medium was replaced with pre-warmed XF Seahorse medium supplemented with glucose, sodium pyruvate and glutamine (all purchased from Agilent, Santa Clara, California, USA)