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HEK293T cells were transfected with transfer plasmid, psPAX2 (Addgene 12260), and pMD2.G (Addgene 12259) at 3.5:3.5:1 ratio using Lipofectamine 2000 according to the manufacturers instruction

This therapeutic effect has also been explored in patients with obesity and major depressive disorder, a population often affected by overlapping pathophysiological processes such as insulin resistance, inflammation, and altered reward system function [33,42,70]

Open 6 , e230524 (2023)

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