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Thus, to reduce Cd accumulation in the leaves without developmental impairment, it was proposed to combine a nonsense mutation in one of the NtHMA4 homologs, which causes a complete loss of function, with a missense mutation in the other NtHMA4 gene causing functional reduction

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Vandrey R, Stitzer ML, Mintzer MZ, Huestis MA, Murray JA, Lee D (2013)