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Chromatography was performed on a 2.1 150 mm, 1.7 m, BEH C18 column (Waters) at 45 C using aqueous HCOOH (0.1% in H 2 O) as solvent A and MeCN containing 0.1% HCOOH as solvent B and the following gradient (flow rate: 0.4 mL/min): For dipeptide analysis in the hydrolysates, chromatography started with 1% solvent B for 2.5 min, which was increased to 95% within 5.5 min, hold for 1.5 min and, then, decreased again to 1% within 0.5 min

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10.1186/1423-0127-17-S1-S23 J

Arginase expression or activity increases in the presence of exogenous arginine (Ide et al., 2020) In the arginase pathway, ornithine and urea are formed from arginine

For MS-based analysis, subsequent wash steps were performed on a Kingfisher Flex equipped with a 96-well Deep Well head