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ROS are toxic to Hessian fly larvae and can also participate in cell wall strengthening 27,28

For SH-SY5Y/MYCN, the culture media was supplemented with 7.5 g/mL Blasticidin (Bio-Sciences) and 200 g/mL Geneticin (G418) (Thermo Scientific) for selection of the plasmid

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Upon ligand activation, these nuclear ERs dimerize and translocate to the nucleus where they act as transcription factors by binding to estrogen response elements directly or interact with coactivators such as activator protein 1 or specificity protein 1, to regulate gene expression (19)

Always inject the bacteriostatic water slowly down the inside glass wall of the vial

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