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E-cigarettes can damage cells related to innate and adaptive immunity in the respiratory tract, resulting in abnormal mucus composition, reduced epithelial barrier function, impaired phagocytic function, and elevated systemic inflammatory markers (especially type 2 inflammatory cytokines IL-4, IL-5, IL-13)

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Only high-quality reads, as measured by the Q20, Q30, and GC content metrics, were retained for downstream analyses