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Initial bacterial attachment assays Bacteria strains were cultured in TSB with or without 2 mg/mL nicotine to mid-exponential phase, and the bacterial cells were diluted with TSB to OD 600 = 0.1
Often used to classify pod kits
They include the following: Higher costs for tobacco products (for example, through increased taxes)2,10,11 Prohibiting smoking in indoor areas of worksites and public places2,10,11 Raising the minimum age of sale for tobacco products to 21 years, which has recently emerged as a potential strategy for reducing youth tobacco use11 TV and radio commercials, posters, and other media messages targeted toward youth to counter tobacco product advertisements2,10 Community programs and school and college policies and interventions that encourage tobacco-free environments and lifestyles2,10 Community programs that reduce tobacco advertising, promotions, and availability of tobacco products2,10 Some social and environmental factors have been found to be related to lower smoking levels among youth
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