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The most common methods of smoking tobacco include manufactured and hand-rolled cigarettes and cigars (worldwide, Europe, and Asia), waterpipes (Middle East, North Africa, and parts of Asia), pipes (worldwide), and more recently electronic nicotine delivery systems (North America and Europe) (Asma et al

It is a cigarette targeting young women, who used to be Marlboro's main customer base, and it has a flavor close to peppermint flavor mixed with sweetness, rather than the common menthol flavor

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The thermal cycling program consisted of an initial denaturation step at 95 C for 8 min, followed by 40 cycles of 95 C for 15 s, 58.9 C for 30 s, and 72 C for 30 s, concluding with a melting curve analysis