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There are rites which must be performed

doi: 10.1038/s41392-024-01839-8 Summary Keywords epithelial cells, innate immunity, kidney-lung crosstalk, mitochondrial quality control, mitophagy, organ crosstalk Citation Zhou X, Sun J, Miao L and Zhang Y (2026) Mitophagy in kidney and lung epithelial cells: molecular mechanisms, crosstalk, and therapeutic interventions

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In my eyes, yes, we should

For Glycolytic Rate Assay, the medium was exchanged to XF DMEM medium (Agilent Technologies) with 2 mM glutamine, 10 mM glucose, 1 mM pyruvate, and 5 mM HEPES and incubated at 37 C without CO 2 1 h prior to the assay