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The V3-V4 region of the bacterial 16S rRNA gene was amplified with the primers 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3), and the ITS1 region of fungi was amplified using ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3) and ITS2R (5-GCTGCGTTCTTCATCGATGC-3) [18, 26]

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