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In addition to the fact that the majority of companies at TPE do not sell premium cigars, the other notable exception is that this is a privately-run, for-profit trade show, as opposed to the PCAs trade show, which is largely used to generate funds for the not-for-profit trade association

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Cells were immunoprecipitated with Sp1 or SIRT1 antibodies or normal IgG (Cell Signaling Technology) and then amplified by PCR