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Briefly, the mainstream smoke of two cigarettes (North State) without a filter was bubbled through 40 mL of culturing medium with an evacuation ejector (AGA MS-33, Solna, Sweden)
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Accessed 08 Aug 2022 OConnor RJ, Bansal-Travers M, Cummings KM, Hammond D, Thrasher JF, Tworek C (2015) BMC Public Health 15:1279 Pauly JL, Stegmeier SJ, Mayer AG, Lesses JD, Streck RJ (1997) Tob Control 6:33 Garner WW, Bacon CW, Bowling JD (1934) Ind Eng Chem 26:970 Workman J, Mark H (2006) Spectroscopy 21:19 Acknowledgements The research was funded with financial support from Faculty of Chemistry, Gdask University of Technology
Early industrial Pittsburgh was also renowned for its manufacturing of cigars
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