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Fibroblasts were first thawed and then seeded at 4 10 3 cells/cm 2 in 75 cm 2 cell culture flasks, while keratinocytes were thawed and seeded at 4 10 3 cells/cm 2 in 75 cm 2 cell culture flasks on a feeder layer of gamma-irradiated human dermal fibroblasts

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When a cigarette is smoked, nicotine is inhaled through the lungs and reaches the brain within seconds, stimulating the production of dopamine, a neurotransmitter associated with the sensation of pleasure

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