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On day 7, the cell medium was replaced by an assay medium consisting of XF Base Medium supplemented with 10 mM glucose, 10 mM pyruvic acid, and 1 mM L-glutamine incubated in a CO 2 -free incubator 1 h before the experiment

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It's important to mention that this is all speculative due to the difficulty of quantifying the two very different types of nicotine inhalation

Let it sit for four to eight hours before vacuuming it away

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