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[DOI] [PMC free article] [PubMed] [Google Scholar] 125.Arosio M., Ronchi C.L., Beck-Peccoz P., Gebbia C., Giavoli C., Cappiello V., Conte D., Peracchi M

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This technique can provide rapid relief when performed correctly, but it should ideally be demonstrated and approved by a healthcare professional before home use

coli culture was collected by centrifugation for 10 min at 3500 rpm and 4 C, washed with ice-cold PBS at pH 7.3, and lysed in 25 ml of lysis buffer (PBS, pH 7.3