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If not, the damage is permanent

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To get a medium grind, you can use a weed grinder with larger holes or make sure that you only turn the grinder a few times

The study was led by first author Ashley Merianos, PhD, University of Cincinnati, and senior author Melinda Mahabee-Gittens, MD, PhD, Division of Emergency Medicine at Cincinnati Childrens

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After homogenization, the sample was incubated in a 90C water bath for 15 min, after which 1 L was transferred to the PCR reaction mixture for amplification