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About this article Cite this article Han, H., Yao, J., Wu, J

after homogenization and lysis, centrifuge at 10,000 g for 10-15 min at 4C, take the supernatant and the protein removal reagent 1:1 and mix thoroughly

In aqueous solutions, especially at physiological temperatures (37C), L-glutamine can spontaneously break down into ammonia and pyrrolidone carboxylic acid (Figure 1)

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