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A 25 L reaction mixture was prepared, comprising 12.5 L PCR Master Mix (2X) (Thermo Scientific), 0.8 L of 10 mM forward primer, 0.8 L of 10 mM reverse primer, 2 L MT-DNA (20 ng/L), and 8.9 L nuclease-free water

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It is also more discreet than combustible tobacco products since you only have to place it in your mouth and enjoy its content.This particular smokeless tobacco now comes in many strengths, with some of them boasting high nicotine concentrations to cater users who prefer a strong buzz

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