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While the study did not assess the levels of urine cotinine or carboxyhemoglobin, we selected CO as a biological marker because it is simple and quick to measure, less expensive than a battery of laboratory tests, and noninvasive, making it more agreeable to the subjects as reported by Middleton et al

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The current results thus indicated that reduced GMV in the left vmPFC could be an inheritable factor of rule-breaking behaviour, such as conduct problems and early substance use, especially in pre-adult

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