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1 and will be implemented through a phased approach

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The supernatant was also saved (tube 2) and the loosened epithelium was then manually scraped off into stock solution with 10% FBS (tube 3), and all cells were collected by centrifugation for 10 min at 225 g , 4 C (tubes 1, 2, and 3)

Abbreviations TP: Tobacco powder TL: Tobacco leaves TSNA: Tobacco-specific nitrosamines B1: Bacillus subtilis B1 C4: Cytobacillus oceanisediminis C4 CMC-Na: Carboxymethylcellulose sodium PDA: Potato dextrose agar PCR: Polymerase chain reactions NCBI: National Center for Biotechnology Information BLAST: Basic local alignment search tool HnB: Heat-not-burn SDE: Simultaneous distillation extraction SEM: Scanning electron microscope XRD: X-ray diffraction FTIR: Fourier transform infrared spectroscopy PCoA: Principle coordination analysis LDA: Linear Discriminate Analysis LEfSe: LDA effect size C4.TP: Cytobacillus oceanisediminis C4.tobacco powder C4.TL: Cytobacillus oceanisediminis C4.tobacco leaves B1.TP: Bacillus subtilis B1.tobacco powder B1.TL: Bacillus subtilis B1.tobacco leaves NMDS: Non-metric multidimensional scaling References Alagi S, Selekcija IS, Pali R, Stojanovi G, Nikoli M (2002) Chemical composition and antimicrobial activity of the essential oil of the oriental tobacco Yaka

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Each carton contains 10 packs for a total of 200 cigarettes, making it a convenient bulk purchase for regular smokers