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Briefly, 15 l of plasma was transferred to a 1.5 mL microcentrifuge tube followed by addition of dH 2 O (200 L, 0.05% formic acid [FA]) containing cotinine- d 3 (250 nM), followed by dilution with ammonium acetate (10 mM, 200 L)

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95% CI, 2.26-5.41]. Other contributors to persistent e-cigarette use included restrictions regarding secondhand exposure to tobacco products and disagreement with the FDAs ruling

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