Single-cell RNA sequencing and mass cytometry analyses of peripheral blood from smokers have demonstrated expansion of differentiated CD16 + CD8 + T-cell populations, reduced nave T-cell representation, and transcriptional programs consistent with altered cytotoxic function and immune aging (64)
[48] The single-nucleotide polymorphisms (SNPs) rs6474413 and rs10958726 in CHRNB3 are highly correlated with nicotine dependence
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