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In addition for lungs, one lobe was stored in RNA Later (Ambion, ThermoFisher Scientific, Illkirch, France) for transcriptomic analyses, posterior lobes were fixed with paraformaldehyde (PFA 4%, Labonord, Villeneuve dAscq, France) for histopathological analyses, and another lobe was frozen in liquid nitrogen and then stored at 80 C until further analyses